inhouse-developed program with matlab software Search Results


inhouse developed software filter  (MathWorks Inc)
90
MathWorks Inc inhouse developed software filter
Inhouse Developed Software Filter, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inhouse developed software filter/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
inhouse developed software filter - by Bioz Stars, 2026-04
90/100 stars
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inhouse code  (MathWorks Inc)
90
MathWorks Inc inhouse code
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Inhouse Code, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inhouse code/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
inhouse code - by Bioz Stars, 2026-04
90/100 stars
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inhouse developed algorithm r2021a  (MathWorks Inc)
90
MathWorks Inc inhouse developed algorithm r2021a
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Inhouse Developed Algorithm R2021a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inhouse developed algorithm r2021a/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
inhouse developed algorithm r2021a - by Bioz Stars, 2026-04
90/100 stars
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matlab r2023a  (MathWorks Inc)
90
MathWorks Inc matlab r2023a
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Matlab R2023a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matlab r2023a/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
matlab r2023a - by Bioz Stars, 2026-04
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matlab code r2017a  (MathWorks Inc)
90
MathWorks Inc matlab code r2017a
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Matlab Code R2017a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matlab code r2017a/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
matlab code r2017a - by Bioz Stars, 2026-04
90/100 stars
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matlab-based program 2018a  (MathWorks Inc)
90
MathWorks Inc matlab-based program 2018a
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Matlab Based Program 2018a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matlab-based program 2018a/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
matlab-based program 2018a - by Bioz Stars, 2026-04
90/100 stars
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software matlab r2018a  (MathWorks Inc)
90
MathWorks Inc software matlab r2018a
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Software Matlab R2018a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software matlab r2018a/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
software matlab r2018a - by Bioz Stars, 2026-04
90/100 stars
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inhouse script  (MathWorks Inc)
90
MathWorks Inc inhouse script
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Inhouse Script, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inhouse script/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
inhouse script - by Bioz Stars, 2026-04
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crysiv matlab based toolbox  (MathWorks Inc)
90
MathWorks Inc crysiv matlab based toolbox
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Crysiv Matlab Based Toolbox, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crysiv matlab based toolbox/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
crysiv matlab based toolbox - by Bioz Stars, 2026-04
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inhouse version of this instrument  (MathWorks Inc)
90
MathWorks Inc inhouse version of this instrument
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Inhouse Version Of This Instrument, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inhouse version of this instrument/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
inhouse version of this instrument - by Bioz Stars, 2026-04
90/100 stars
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matlab 2014a  (MathWorks Inc)
90
MathWorks Inc matlab 2014a
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Matlab 2014a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matlab 2014a/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
matlab 2014a - by Bioz Stars, 2026-04
90/100 stars
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inhouse developed software  (MathWorks Inc)
90
MathWorks Inc inhouse developed software
A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in <t>MATLAB.</t> Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.
Inhouse Developed Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inhouse developed software/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
inhouse developed software - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in MATLAB. Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.

Journal: bioRxiv

Article Title: Mechanical stress in pancreatic cancer: Signaling pathway adaptation activates cytoskeletal remodeling and enhances cell migration

doi: 10.1101/2021.06.11.448065

Figure Lengend Snippet: A, MIA PaCa-2 and PANC-1 cells were pre-treated with 15 μM p38 MAPK inhibitor (SB202190), JNK inhibitor (SP600125) or equal volume of DMSO and subjected to a scratch wound healing assay under 4.0 mmHg of compression. Pictures from at least 4 different fields were taken from three biological replicates. Scale bar: 0.2 mm. White dashed line shows the difference in wound closure between 0 and 16 hours. B-C, Graph showing the average ±SE percentage wound closure of compressed MIA PaCa-2 (B) and PANC-1 (C) treated with DMSO or inhibitors from 3 biological replicates (n≥12). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). D-E , Graph showing the average Ki67 area fraction in compressed MIA PaCa-2 (D) and PANC-1 (E) cells treated with DMSO, p38 MAPK or JNK inhibitor from at least 10 different fields/ condition from two biological replicates as quantified automatically using an in-house code in MATLAB. Asterisk (*) indicates a statistically significant difference in student’s t test (p<0.05). F , Representative Western Blotting showing phosphorylated HSP27 (Ser 82) and c-Jun (S73) in control and compressed MIA PaCa-2 cells treated with 15 μM of each inhibitor or equal volume of DMSO. Antibody against β-actin was used as a loading control. Quantification of each antibody compared to loading control was quantified by ImageJ and it is indicated by numbers in grey font.

Article Snippet: For image analysis, the calculation of Ki67 area fraction was performed automatically using a previously developed inhouse code in MATLAB (MathWorks, Inc., Natick,MA) , , .

Techniques: Wound Healing Assay, Western Blot, Control

A-B , Rac1 (A) and cdc42 (B) mRNA expression was quantified by qPCR in both MIA PaCa-2 and PANC-1. Each bar indicates the mean fold change ±SE of two biological replicates (n=6). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). C , MIA PaCa-2 and PANC-1 pancreatic cancer cells were treated with siRNA against Rac1 (siRac1) or cdc42 (sicdc42) and then subjected to a scratch wound healing assay for 16 hours under 0.0 or 4.0 mmHg of compression in 2 % FBS containing DMEM. Control cells were treated with stealth siRNA (siCTRL). Scale bar: 0.1 mm. D-E , Graphs showing the percentage ±SE wound closure of MIA PaCa-2 (D) and PANC-1 (E) as quantified using ImageJ software. Statistically significant difference in wound closure of compressed siRac1- or sicdc42-treated cells compared to siCTRL-treated cells is indicated with an asterisk (*) (2 biological replicates; n≥6; p<0.05 in student’s t test). F , Graph showing the average ±SE Ki67 area fraction in MIA PaCa-2 and PANC-1 control and compressed cells treated with siCTRL, siRac1 or sicdc42 from at least 5 different fields/ condition from two biological replicates as quantified automatically using an in-house code in MATLAB. No statistically significant changes were observed.

Journal: bioRxiv

Article Title: Mechanical stress in pancreatic cancer: Signaling pathway adaptation activates cytoskeletal remodeling and enhances cell migration

doi: 10.1101/2021.06.11.448065

Figure Lengend Snippet: A-B , Rac1 (A) and cdc42 (B) mRNA expression was quantified by qPCR in both MIA PaCa-2 and PANC-1. Each bar indicates the mean fold change ±SE of two biological replicates (n=6). Asterisk (*) indicates a statistically significant difference (p<0.05 in student’s t test). C , MIA PaCa-2 and PANC-1 pancreatic cancer cells were treated with siRNA against Rac1 (siRac1) or cdc42 (sicdc42) and then subjected to a scratch wound healing assay for 16 hours under 0.0 or 4.0 mmHg of compression in 2 % FBS containing DMEM. Control cells were treated with stealth siRNA (siCTRL). Scale bar: 0.1 mm. D-E , Graphs showing the percentage ±SE wound closure of MIA PaCa-2 (D) and PANC-1 (E) as quantified using ImageJ software. Statistically significant difference in wound closure of compressed siRac1- or sicdc42-treated cells compared to siCTRL-treated cells is indicated with an asterisk (*) (2 biological replicates; n≥6; p<0.05 in student’s t test). F , Graph showing the average ±SE Ki67 area fraction in MIA PaCa-2 and PANC-1 control and compressed cells treated with siCTRL, siRac1 or sicdc42 from at least 5 different fields/ condition from two biological replicates as quantified automatically using an in-house code in MATLAB. No statistically significant changes were observed.

Article Snippet: For image analysis, the calculation of Ki67 area fraction was performed automatically using a previously developed inhouse code in MATLAB (MathWorks, Inc., Natick,MA) , , .

Techniques: Expressing, Wound Healing Assay, Control, Software